| Enzyme | Sequence | Isoschizomer |
|---|---|---|
| Aat II | GACGT|C | Ssp5230 I |
| Acc II | CG|CG | Bpu95 I, Bsh1236 I, BstFN I, BstU I, Bsu1532 I, Btk I, FnuD II, Mvn I, Tha I |
| Acl I | AA|CGTT | Psp1406 I |
| Afe I | AGC|GCT | Aor51H I, Eco47 III |
| Afl II | C|TTAAG | Bfr I, BspT I, Bst98 I, Esp4 I, MspC I, Vha464 I |
| Age I | A|CCGGT | |
| Alu I | AG|CT | Mlt I |
| Apa I | GGGCC|C | |
| ApaL I | G|TGCAC | Alw44 I, Sno I, Uba44 I, Vne I |
| Asc I | GG|CGCGCC | |
| Ase I | AT|TAAT | Asn I, PshB I, Vsp I |
| AsiS I | GCGAT|CGC | Sgf I |
| Ava III | ATGCA|T | EcoT22 I, Mph1103 I, Nsi I, PinB I, Sep I, Zsp2 I |
| Avr II | C|CTAGG | AvrB II, Bln I, Bsp2A I |
| Bal I | TGG|CCA | Mls I, MluN I, Mlu31 I, Msc I, Msp20 I |
| BamH I | G|GATCC | AccEB I, Ali II, Bce751 I, Bna I, Bst I, Mlu23 I, NspSA IV, Sur I |
| Bbe I | GGCGC|C | |
| BbvC I | CC|TCAGC | |
| Bcl I | T|GATCA | BsiQ I, BspX II, Fba I, Ksp22 I, Pov I |
| BfrB I | ATG|CAT | |
| Bgl II | A|GATCT | Ncr I, NspMAC I, Pae2k I, Pae18k I |
| Bma I | CGA|TCG | Afa16R I, Afa22M I |
| Bmt I | GCTAG|C | Ace II |
| BsiW I | C|GTACG | BpuB5 I, Pfl23 II, PpuA I, PspL I, Spl I, Sun I |
| BspE I | T|CCGGA | Acc III, Aor13H I, BbvA III, BseA i, BsiM I, BspM I, Bsp13 I, Bsu23 I, CauB3 I, Kpn2 I, Mro I, PinB II, Pta I |
| BspH I | T|CATGA | Pag I, Rca I |
| BspKT6 I | GAT|C | |
| BsrG I | T|GTACA | Aau I, Bsp24 I, SspB I |
| BssH II | G|CGCGC | BseP I, Pau I |
| BstB I | TT|CGAA | Asu II, Acp I, Asp10H I, Bim I, Bim19 I, Bpu14 I, BsiC I, BspT104 I, Bsp119 I, Cbi I, Csp45 I, Fsp II, Lsp I, Mla I, Nsp V, Pla II, Sfu I, SspRF I, Ssp1 I, Ssp152 I |
| Bsu90 I | GG|ATCC | |
| BstZ17 I | GTA|TAC | |
| Cla I | AT|CGAT | Aag I, Ban III, BbvA II, Bsi29 I, Bdi I, Bli41 I, Bli86 I, Bsa29 I, Bsc I, BseC I, BsiX I, BspD I, BspJ II, BspX I, Bsp106 I, Bsu15 I, Lpl I, Pga I |
| Cha I | GATC| | |
| CviA II | C|ATG | |
| CviQ I | G|TAC | Csp6 I, CviR II |
| Dpn I | GA|TC | |
| Dra I | TTT|AAA | Aha III |
| Eag I | C|GGCCG | Aaa I, BseX3 I, BsfZ I, EclX I, Eco52 I, Xma III |
| EcoR I | G|AATTC | Hal I, Kpn49k I, Rsr I, Sso I |
| EcoR V | GAT|ATC | Ceq I, Eco32 I |
| Fat I | |CATG | |
| Fse I | GGCCGG|CC | |
| Fsp I | TGC|GCA | Acc16 I, Aos I, Avi II, Fdi II, Fsp I, Mst I, Nsb I, Pam I |
| Hae III | GG|CC | Bim19 II, BseQ I, Bsh I, BspBR I, BspK I, BspR I, Bsp211 I, BsuR I, Clt I, Dsa II, FnuD I, MfoA I, Pal I, Pde133 I, PflK I, Pho I, Pla I, Sbv I, Sfa I, Sua I |
| Hap II | C|CGG | Bco27 I, BsiS I, Bst40 I, Hpa II, Hin2 I, Mno I, Msp I, Pde137 I, Sth134 I |
| Hha I | GCG|C | AspLE I, Cfo I, FnuD III |
| Hind III | A|AGCTT | BstF I, Eco VIII, Hsu I, Ssb I |
| HinP1 I | G|CGC | Hin6 I, HspA I, SciN I |
| Hpa I | GTT|AAG | |
| HpyCH4 IV | A|CGT | |
| HpyCH4 V | TG|CA | CviR I |
| Kas I | G|GCGCC | |
| Kpn I | GGTAC|C | Acc65 I, AhaB8 I, Asp718 I, Sth I |
| Mfe I | C|AATTG | Mun I |
| Mlu I | A|CGCGT | |
| Mlu113 I | CC|GCGG | |
| Mrh I | CT|CGAG | MlaA I |
| Mse I | T|TAA | Tru1 I, Tru9 I |
| Mxa I | GAG|CTC | Ecl136 I, EcoICR I |
| Nae I | GCC|GGC | SauBMK I, SauHP I, SauLP I, SauN I, SauS I, Slu1777 I |
| Nar I | GG|CGCC | Mch I, Nda I, Nun II, SseA I |
| Nco I | C|CATGG | Bsp19 I |
| Nde I | CA|TATG | FauND I |
| NgoM IV | G|CCGGC | Eco56 I, MroN I, NgoA IV, NgoF IV, NgoM I |
| Nhe I | G|CTAGC | PstNH I |
| Nla III | CATG| | Hsp92 II |
| Not I | GC|GGCCGC | |
| Nru I | TCG|CGA | Bsp68 I, MluB2 I, Sbo13 I, Spo I |
| Pac I | TTAAT|TAA | |
| Pci I | A|CATGT | BspLU11 I |
| PmaC I | CAC|GTG | BbrP I, BcoA I, Eco72 I, Pml I |
| Pme I | GTTT|AAAC | Mss I |
| Ppu10 I | A|TGCAT | |
| Psi I | TTA|TAA | |
| PspOM I | G|GGCCC | Bsp120 I |
| Pst I | CTGCA|G | AliAJ I, Api I, Asp713 I, BspB I, Bsp63 I, Cfl I, CfrA4 I, Cfu II, Cst I, Hal II, MhaA I, PaeP I, Pma44 I, SalP I, Sfl I, Yen I |
| Pvu I | CGAT|CG | BspC I, ErhB9 I, Nbl I, Ple19 I, Psu161 I, Rsh I, Xor II |
| Pvu II | CAG|CTG | Bav I, BavA I, BavB I, BspO4 I, Cfr6 I, Dma I, Pvu84 II |
| Rsa I | GT|AC | Afa I |
| Sac I | GAGCT|C | Psp124B I, Sst I |
| Sac II | CCGC|GG | Cfr42 I, Csc I, Eae46 I, Eco29k I, Gal I, Gce I, GceGL I, Kpn19 I, Ksp I, NgoA III, NgoF III, NgoP III, PaeA I, Sfr303 I, SgrB I, Sst II |
| Sal I | G|TCGAC | HgiC III, HgiD II, Nop I, Rtr I, Xci I |
| Sau3A I | |GATC | AspMD I, Bce243 I, BfuC I, BspA I, BspF I, BspJ I, Bsp67 I, Bsp105 I, Bsp143 I, Bsp2095 I, Btk II, Cac I, Ccy I, Cpf I, CviA I, Dpn II, FnuC I, FnuE I, Hac I, Kzo9 I, LlaA I, Mbo I, Nde II, Nla II, NmeC I, Nph I |
| Sbf I | CCTGCA|GG | Sda I, Sse8387 I |
| Sca I | AGT|ACT | Acc113 I, Dpa I, Eco255 I, RflF II |
| Sci I | CTC|GAG | |
| Sel I | |CGCG | |
| Sfo I | GGC|GCC | Pmn I |
| Sma I | CCC|GGG | CfrJ4 I, PaeB I, PspAL I |
| SnaB I | TAC|GTA | BstSN I, Eco105 I |
| Spe I | A|CTAGT | AclN I, Bcu I |
| Sph I | GCATG|C | Bbu I, Pae I |
| Srf I | GCCC|GGGC | |
| Sse232 I | CG|CCGGCG | |
| Ssp I | AAT|ATT | |
| Stu I | AGG|CCT | Aat I, Eco147 I, Gdi I, Pme55 I, SseB I |
| Swa I | ATTT|AAAT | Smi I |
| Tai I | ACGT| | Tsc I |
| Taq I | T|CGA | TthHB8 I |
| Tsp509 I | |AATT | TspE I, Sse9 I, Tas I |
| Xba I | T|CTAGA | |
| Xho I | C|TCGAG | Abr I, Blu I, Ccr I, Mav I, PaeR7 I, Pan I, Sau3239 I, Sbi68 I, Sfr274 I, Sla I, Sol10179 I, Tli I, Xpa I |
| Xma I | C|CCGGG | Ahy I, Cfr9 I, EaeA I, PspA I, Xcy I, XmaC I |
| Xsp I | C|TAG | Bfa I, Mae I, MthZ I, Rma I |
| Zra I | GAC|GTC |